Product Name	LC3A/B
Chinese Name	自噬微管相关蛋白轻链3A/3B抗体
Alias	MLP3A_HUMAN; Microtubule-associated proteins 1A/1B light chain 3A; MAP1LC3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A (MAP1A/MAP1B LC3 A); Microtubule-associated protein 1 light chain 3 alpha; MLP3B_HUMAN; Microtubule-associated proteins 1A/1B light chain 3B; MAP1LC3B; MAP1ALC3; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B (MAP1A/MAP1B LC3 B); Microtubule-associated protein 1 light chain 3 beta;
literatures	Specific References  (2)     |     SL20414R has been referenced in 2 publications. [IF=7.086] Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625  IF ;  Mouse.   PubMed:34303901 [IF=6.331] Maowen Chen. et al. Enhanced biocompatibility and osteogenic differentiation of mesenchymal stem cells of titanium by Sr–Ga clavate double hydroxides. J Mater Chem B. 2021 Jul;:  IF ;  Rat.   PubMed:34259279
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Cytoskeleton  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Cow, Horse, Sheep, Guinea Pig, Goat, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	14kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LC3A/B: 1-50/121
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts with SQSTM1 (By similarity). Interacts with TP53INP1 and TP53INP2. Subcellular Location: Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes. Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Post-translational modifications: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Similarity: Belongs to the MAP1 LC3 family. SWISS: Q9GZQ8 Gene ID: 81631 Database links: Entrez Gene: 81631 Human Entrez Gene: 84557 Human Entrez Gene: 66734 Mouse Entrez Gene: 67443 Mouse Entrez Gene: 362245 Rat Entrez Gene: 64862 Rat Omim: 601242 Human Omim: 609604 Human SwissProt: Q9GZQ8 Human SwissProt: Q9H492 Human SwissProt: Q91VR7 Mouse SwissProt: Q9CQV6 Mouse SwissProt: Q62625 Rat SwissProt: Q6XVN8 Rat Unigene: 356061 Human Unigene: 196239 Mouse Unigene: 28357 Mouse Unigene: 41412 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A/B) Polyclonal Antibody, Unconjugated (SL20414R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃). Primary Antibody (green line): Rabbit Anti-LC3A/B antibody (SL20414R), Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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