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Product Name Phospho-Cofilin (Ser3) Chinese Name 磷酸化丝切蛋白(Ser3)抗体 Alias Cofilin (Phospho-Ser3); Cofilin (Phospho S3); Cofilin-1; 18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN. literatures Specific References (1) | SL20261R has been referenced in 1 publications.[IF=6.543] Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022 WB,IF ; Rat,Human.Research Area Tumour Cell biology immunology Neurobiology Signal transduction Growth factors and hormones Cell adhesion molecule Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3: A(p-S)GV Lsotype IgG Purification affinity purified by Protein A Buffer Solution Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004].
Function:
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Subunit:
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.
Subcellular Location:
Nucleus matrix. Cytoplasm, cytoskeleton
Tissue Specificity:
Widely distributed in various tissues.
Post-translational modifications:
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
Similarity:
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain.
SWISS:
P23528
Gene ID:
1072
Database links:Entrez Gene: 1072 Human
Entrez Gene: 12631 Mouse
Entrez Gene: 494432 Sheep
Omim: 601442 Human
SwissProt: P23528 Human
SwissProt: P18760 Mouse
SwissProt: Q6B7M7 Sheep
Unigene: 52428 Cow
Unigene: 170622 Human
Unigene: 329655 Mouse
Unigene: 11675 Rat
Unigene: 13212 Sheep
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (SL20261R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (SL20261R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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