Product Name	MAP1A
Chinese Name	微管相关蛋白1A抗体
Alias	MAP 1A; MAP-1A; MTAP1A; MAP 1L; MAP1L; MAP1A heavy chain; Map1a; MAP1A_HUMAN; Microtubule associated protein 1 like; Microtubule Associated Protein 1A; Microtubule-associated protein 1A; MTAP1A; Proliferation related protein p80; Proliferation-related protein p80; AI853608; Map1a; moth1; Mtap-1; Mtap1.
Research Area	Tumour  Cell biology  immunology  Neurobiology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	326kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MAP1A: 2531-2630/2803
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain. Expression of this gene is almost exclusively in the brain. Studies of the rat microtubule-associated protein 1A gene suggested a role in early events of spinal cord development. [provided by RefSeq, Jul 2008] Function: Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements. Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with TIAM2. Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG4. Binds to CSNK1D. Subcellular Location: Cytoplasm, cytoskeleton (Probable). Tissue Specificity: Brain Post-translational modifications: Phosphorylated by CSNK1D. LC2 is generated from MAP1A by proteolytic processing. Similarity: Belongs to the MAP1 family. SWISS: P78559 Gene ID: 4130 Database links: Entrez Gene: 4130 Human Entrez Gene: 17754 Mouse Entrez Gene: 25152 Rat Omim: 600178 Human SwissProt: P78559 Human SwissProt: Q9QYR6 Mouse SwissProt: P34926 Rat Unigene: 194301 Human Unigene: 619338 Human Unigene: 36501 Mouse Unigene: 11402 Rat Unigene: 41412 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1A) Polyclonal Antibody, Unconjugated (SL20255R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1A) Polyclonal Antibody, Unconjugated (SL20255R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1A) Polyclonal Antibody, Unconjugated (SL20255R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control（black line）:U87MG. Primary Antibody (green line): Rabbit Anti-MAP1A antibody (SL20255R) Dilution:1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Negative control（white blue line）: PBS Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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