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Rabbit Anti-Placental alkaline phosphatase (PLAP)antibody
Rabbit Anti-Placental alkaline phosphatase (PLAP)antibody
Alkaline phosphatase; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; ALP; Alp1; ALPP; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; PALP; Placental alkaline phosphata
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  • NO.:SL20015R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,
    Applications:WB ELISA Flow-Cyt
    concentration:1mg/ml
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Product Name Placental alkaline phosphatase (PLAP)
Chinese Name 碱性磷酸酶(胎盘型)抗体
Alias Alkaline phosphatase; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; ALP; Alp1; ALPP; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; placental type; PLAP; PLAP-1; PLAP1; PPB1_HUMAN.  碱性磷酸酶胎盘型; 胎盘碱性磷酸酶;
Research Area Tumour  Kinases and Phosphatases  The new supersedes the old  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse, 
Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 53kDa
Cellular localization The cell membrane 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human Placental alkaline phosphatase 1: 41-140/535 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Subunit:
Homodimer.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

Similarity:
Belongs to the alkaline phosphatase family.

SWISS:
P05187

Gene ID:
250

Database links:

Entrez Gene: 250 Human

Omim: 171800 Human

SwissProt: P05187 Human

Unigene: 284255 Human



Product Picture
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Placental alkaline phosphatase (SL20015R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-Placental alkaline phosphatase antibody (SL20015R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.

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