TEL: +86 571 56623320 EMAIL: [email protected]
Product Name PASK/STK37 Chinese Name 丝氨酸/苏氨酸蛋白激酶37抗体 Alias DKFZP434O051; DKFZp686P2031; hPASK; KIAA0135; MGC93882; mKIAA0135; PAS domain containing serine/threonine kinase; PAS domain containing serine/threonine protein kinase; PAS domain-containing serine/threonine-protein kinase; PAS kinase; PAS serine/threonine kinase; PAS-kinase; Pask; PASK_HUMAN; PASKIN; STK 37; STK37. Research Area Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 143kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PASK/STK37: 31-130/1323 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Function:
Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
Post-translational modifications:
Autophosphorylated on Thr-1161 and Thr-1165.
Similarity:
Belongs to the protein kinase superfamily.
CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 protein kinase domain.
SWISS:
Q96RG2
Gene ID:
23178
Database links:Entrez Gene: 23178 Human
Entrez Gene: 269224 Mouse
Omim: 607505 Human
SwissProt: Q96RG2 Human
SwissProt: Q8CEE6 Mouse
Unigene: 397891 Human
Unigene: 379454 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PASK) Polyclonal Antibody, Unconjugated (SL19896R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Scan Wechat Qrcode