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Product Name SLC32A1 Chinese Name 溶质载体家族蛋白32成员A1抗体 Alias bA122O1.1; GABA and glycine transporter; hVIAAT; SLC32A 1; Slc32a1; solute carrier family 32 (GABA vesicular transporter) member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN. Research Area Cell biology Neurobiology Signal transduction Transporter Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SLC30A9: 351-450/525 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008]
Function:
Involved in the uptake of GABA and glycine into the synaptic vesicles.
Subcellular Location:
Cytoplasmic vesicle membrane.
Tissue Specificity:
Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
Similarity:
Belongs to the amino acid/polyamine transporter 2 family.
SWISS:
Q9H598
Gene ID:
140679
Database links:Entrez Gene: 140679 Human
Entrez Gene: 22348 Mouse
SwissProt: Q9H598 Human
SwissProt: O35633 Mouse
Unigene: 179080 Human
Unigene: 143404 Mouse
Unigene: 413854 Mouse
Unigene: 10846 Rat
Product Picture Sample:heart (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1(SL19821R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 60kD
Sample:Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1(SL19821R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated(SL19821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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