Product Name	RBM22
Chinese Name	RNABinding protein22抗体
Alias	Cwc2; FLJ10290; fSAP47; Functional spliceosome associated protein 47; Pre mRNA splicing factor RBM22; Pre-mRNA-splicing factor RBM22; RBM 22; rbm22; RBM22_HUMAN; RNA binding motif protein 22; RNA-binding motif protein 22; ZC3H16; Zinc finger CCCH domain containing protein 16; Zinc finger CCCH domain-containing protein 16.
Research Area	Cell biology  transcriptional regulatory factor  Binding protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	47kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RBM22: 101-300/420
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an RNA binding protein. The encoded protein may play a role in cell division and may be involved in pre-mRNA splicing. Related pseudogenes exist on chromosomes 6, 7, 9, 13, 16, 18, and X. [provided by RefSeq, Mar 2009] Function: Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses. Subcellular Location: Nucleus. Cytoplasm. Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol. Similarity: Belongs to the SLT11 family. Contains 1 C3H1-type zinc finger. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q9NW64 Gene ID: 55696 Database links: Entrez Gene: 512330 Cow Entrez Gene: 489184 Dog Entrez Gene: 55696 Human Entrez Gene: 66810 Mouse Entrez Gene: 100345063 Rabbit Entrez Gene: 307410 Rat Omim: 612430 Human SwissProt: Q3B7L8 Cow SwissProt: Q9NW64 Human SwissProt: Q8BHS3 Mouse SwissProt: Q4V7D7 Rat Unigene: 713564 Human Unigene: 275106 Mouse Unigene: 105130 Rat
Product Picture	Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-RBM22 (SL19767R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM22) Polyclonal Antibody, Unconjugated (SL19767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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