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Product Name KLK1 Chinese Name 激肽释放酶1抗体 Alias Kallikrein 1; Kallikrein-1; hK1; Klk6; KLKR; Glandular kallikrein 1; hK 1; Kallikrein serine protease 1; Kidney/pancreas/salivary gland kallikrein; Klk 6; Tissue kallikrein; Klk1b3; Klk1c1; Ngfg; rGK-1; preprokallikrein; KLK1_HUMAN. literatures Specific References (1) | SL1963R has been referenced in 1 publications.[IF=0] Morrison MD et al. Identifying Candidate Biomarkers for Pleomorphic Adenoma: A Case-Control Study. Head Neck Pathol. 2019 Sep;13(3):286-297. IHC-P ; Human.Research Area Tumour Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 26kDa Cellular localization The nucleus Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human KLK1: 101-200/262 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]
Function:
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Tissue Specificity:
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Post-translational modifications:
The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
Similarity:
Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.
SWISS:
P06870
Gene ID:
3816
Database links:Entrez Gene: 3816 Human
GenBank: NP_002248.1 Human
Omim: 147910 Human
SwissProt: P06870 Human
Unigene: 123107 Human
Unigene: 11331 Rat
激肽释放酶KLK是激肽系统的主要限速酶,它是一组存在于多数组织和体液中的丝氨酸蛋白酶,是一种肽链内切酶。KLK又称血管舒缓素,包括15个家族成员。在不同的组织中广泛表达,具有蛋白水解酶的活性。它特异性的在碳末端切割底物肽,可裂解激肽原释放具有活性的激肽,由激肽发挥对Cardiovascular系统及肾脏功能的调节作用。
组织KLK是一个大的基因家族,主要分布在肺、肾、血管、脑、肾上腺组织,为一种中等大小的glycoprotein。Product Picture Sample:
Lane 1: Human K562 cell lysates
Lane 2: Human SW480 cell lysates
Primary: Anti-KLK1 (SL1963R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (SL1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (SL1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (SL1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (SL1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (SL1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (SL1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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