Product Name	WNT2B
Chinese Name	信号通路Wnt2B抗体
Alias	wingless-type MMTV integration site family, member 2B; XWNT2; WNT13; WNT2B_HUMAN.
literatures	Specific References  (3)     |     SL1946R has been referenced in 3 publications. [IF=5.738] Jianglin Wang. et al. Validation and analysis of expression, prognosis and immune infiltration of WNT gene family in non-small cell lung cancer. FRONT ONCOL. 2022; 12: 911316  WB, IHC ;  Human.   PubMed:35957916 [IF=5.06] Li, Guo, et al. "MicroRNA-324-3p regulates nasopharyngeal carcinoma radioresistance by directly targeting WNT2B." European Journal of Cancer (2013).  WB ;   PubMed:23583221 [IF=4.486] Xu T et al. MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med . 2020 Jul;24(13):7127-7140.  WB ;  Human.   PubMed:32463570
Research Area	Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human WNT2B: 301-391/391
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	WNT2B is a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis. This gene produces two alternative transcript variants.This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis. This gene produces two alternative transcript variants. Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines. Post-translational modifications: Palmitoylation at Ser-243 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-107. Palmitoylation is necessary for proper trafficking to cell surface (By similarity). Similarity: Belongs to the Wnt family. SWISS: Q93097 Gene ID: 7482 Database links: Entrez Gene: 445420 Cow Entrez Gene: 7482 Human Entrez Gene: 22414 Mouse Entrez Gene: 116466 Rat Omim: 601968 Human SwissProt: Q93097 Human SwissProt: O70283 Mouse Unigene: 27254 Cow
Product Picture	Protein: lung(mouse) lysates at 30ug; liver(mouse) lysates at 30ug; Primary: Anti-WNT2B(SL1946R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 44kD Observed band size : 48kD Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Heart tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Heart tissue lysates Primary: Anti-WNT2B (SL1946R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 47 kDa Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT2B) Polyclonal Antibody, Unconjugated (SL1946R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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