Product Name	WRCH1
Chinese Name	WRCH1抗体
Alias	WRCH-1; WRCH 1; Wnt-1 responsive Cdc42 homolog 1; Rho-related GTP-binding protein RhoU; Ryu GTPase; CDC42-like GTPase 1; Rho GTPase-like protein ARHU; GTP-binding protein-like 1; RHOU; SB128; ARHU; CDC42L1; G28K; WRCH1; RHOU_HUMAN.
Research Area	Cell biology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	29kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human WRCH-1: 151-258/258
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The WRCH1 protein is a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. Function: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Subunit: Interacts with PAK3. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction, focal adhesion. Cell projection, podosome. Note=Localizes to podosomes in SRC-transformed cells. Tissue Specificity: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta. Post-translational modifications: Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation. Similarity: Belongs to the small GTPase superfamily. Rho family. SWISS: Q7L0Q8 Gene ID: 58480 Database links: Entrez Gene: 58480 Human Entrez Gene: 69581 Mouse Omim: 606366 Human SwissProt: Q7L0Q8 Human SwissProt: Q9EQT3 Mouse Unigene: 647774 Human Unigene: 168257 Mouse
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- WRCH1 (SL1944R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated (SL1944R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated (SL1944R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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