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Product Name NME3 Chinese Name 二磷酸核苷激酶3抗体 Alias DR-nm23; Expressed in non metastatic cells 3; NDK 3; NDK3; NDK3_HUMAN; NDP kinase 3; NDP kinase C; NDPK C; NDPKC; NM23 H3; nm23-H3; NME 3; Nme3; Non metastatic cells 3 protein expressed in; Nucleoside diphosphate kinase 3; Nucleoside diphosphate kinase C. Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Pig, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 19kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NME3: 81-169/169 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NME3) Polyclonal Antibody, Unconjugated (SL19294R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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