Product Name	MMP8
Chinese Name	基质金属蛋白酶-8/胶原酶2抗体
Alias	Matrilysin/matrix metalloproteinases-8; CLG 1; CLG1; Collagenase II; Collagenase 2; Collagenase neutrophil; HNC; Matrix metalloprotease 8; MMP8; MMP 8; MMP-8; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNLCL; neutrophil collagenase preproprotein; MMP8_HUMAN; Neutrophil collagenase;Matrix metalloproteinase-8; PMNL-CL.
literatures	Specific References  (4)     |     SL1913R has been referenced in 4 publications. [IF=5.246] Yin Zhuang. et al. Exosomes Secreted by Nucleus Pulposus Stem Cells Derived From Degenerative Intervertebral Disc Exacerbate Annulus Fibrosus Cell Degradation via Let-7b-5p. Front Mol Biosci. 2021; 8: 766115  WB ;  Human.   PubMed:35111808 [IF=3.27] Oralova, V., et al. "Osteogenic Potential of the Transcription Factor c-MYB." Calcified Tissue International: 1-12.  WB ;  Mouse.   PubMed:28012106 [IF=3.04] Sîrbulescu, Ruxandra F., et al. "Mature B cells accelerate wound healing after acute and chronic diabetic skin lesions." Wound Repair and Regeneration (2017).  IHC-F ;  Mouse.   PubMed:28922523 [IF=2.35] Zhang, Chao-ying, et al. "Hydrogen sulfide suppresses the expression of MMP-8, MMP-13, and TIMP-1 in left ventricles of rats with cardiac volume overload." Acta Pharmacologica Sinica (2013).  Rat.   PubMed:23974514
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38kDa
Cellular localization	cytoplasmic Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MMP8: 241-340/467
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] Function: Can degrade fibrillar type I, II, and III collagens. Subcellular Location: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix (Probable). Note=Stored in intracellular granules. Tissue Specificity: Neutrophils. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P22894 Gene ID: 4317 Database links: Entrez Gene: 4317 Human Entrez Gene: 17394 Mouse Entrez Gene: 63849 Rat Omim: 120355 Human SwissProt: P22894 Human SwissProt: O70138 Mouse SwissProt: O88766 Rat Unigene: 161839 Human Unigene: 16415 Mouse Unigene: 44474 Rat
Product Picture	Sample: Lane 1: Mouse Bone marrow Lysates Lane 2: Mouse Spleen Lysates Lane 3: Mouse Uterus Lysates Lane 4: Mouse Testis Lysates Lane 5: Mouse NIH/3T3 cell Lysates Lane 6: Rat Bone marrow Lysates Lane 7: Rat Spleen Lysates Lane 8: Rat Uterus Lysates Lane 9: Rat Testis Lysates Lane 10: Human Jurkat cell Lysates Lane 11: Human A549 cell Lysates Lane 12: Human HL-60 cell Lysates Lane 13: Human MCF-7 cell Lysates Lane 14: Human HepG2 cell Lysates Primary: Anti-MMP8 (SL1913R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38kDa Observed band size: 60kDa Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-MMP8 /AF647 Conjugated antibody (SL1913R-AF647) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-AF647 . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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