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Product Name ZFP30 Chinese Name Zinc finger protein30抗体 Alias KIAA0961; ZFP 30; Zfp-30; ZFP30; ZFP30_HUMAN; Zinc finger protein 30 homolog; Zinc finger protein 745; ZNF745. Research Area Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 62kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ZFP30: 101-200/519 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ZFP30 is a 519 amino acid protein that localizes to the nucleus and is composed of thirteen C2H2-type zinc fingers and one KRAB domain. ZFP30 is encoded by a gene located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG families, and Fc receptors (FcRs).
Function:
May be involved in transcriptional regulation.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 13 C2H2-type zinc fingers.
Contains 1 KRAB domain.
SWISS:
Q9Y2G7
Gene ID:
22835
Database links:
Entrez Gene: 22835 Human
Entrez Gene: 22693 Mouse
SwissProt: Q9Y2G7 Human
SwissProt: Q60585 Mouse
Unigene: 716719 Human
Product Picture Sample:
293T(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
Primary: Anti-ZFP30 (SL19124R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Sample:
MDA-MB-231(Human) Cell Lysate at 30 ug
Primary: Anti-ZFP30 (SL19124R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFP30) Polyclonal Antibody, Unconjugated (SL19124R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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