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Product Name MDM1 Chinese Name MDM1蛋白抗体 Alias FLJ95264; MDM 1; Mdm1; Mdm1 nuclear protein homolog (mouse); Mdm1 nuclear protein homolog; MDM1_HUMAN; Mdm4 transformed 3T3 cell double minute 1 p53 binding protein (mouse); Mdm4 transformed 3T3 cell double minute 1 p53 binding protein; Mouse Mdm1 nuclear protein homolog; Nuclear protein double minute 1; Nuclear protein MDM1. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 81kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MDM1: 81-180/714 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Subcellular Location:
Nucleus.
Similarity:
Belongs to the MDM1 family.
SWISS:
Q8TC05
Gene ID:
56890
Database links:Entrez Gene: 56890 Human
Entrez Gene: 17245 Mouse
Entrez Gene: 557148 Zebrafish
Omim: 613813 Human
SwissProt: Q8TC05 Human
SwissProt: Q9D067 Mouse
SwissProt: Q5RHU7 Zebrafish
Unigene: 655702 Human
Unigene: 101191 Mouse
Unigene: 442618 Mouse
Unigene: 162942 Rat
Unigene: 65799 Zebrafish
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MDM1) Polyclonal Antibody, Unconjugated (SL18746R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MDM1) Polyclonal Antibody, Unconjugated (SL18746R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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