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Product Name MAP3K13/LZK Chinese Name MAP3K13蛋白抗体 Alias EC 2.7.11.25; Leucine zipper bearing kinase; Leucine zipper bearing kinase; Leucine zipper-bearing kinase; LZK; M3K13_HUMAN; MAP3K13; MEKK13; MGC133196; Mitogen activated protein kinase kinase kinase 13; Mitogen-activated protein kinase kinase kinase 13; Mixed lineage kinase; MLK; OTTHUMP00000210397. Research Area Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Cow, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 108kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MAP3K13/LZK: 801-900/966 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]
Function:
Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.
Subcellular Location:
Cytoplasm. Membrane.
Tissue Specificity:
Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas.
Post-translational modifications:
Autophosphorylated on serine and threonine residues.
Similarity:
Belongs to the protein kinase superfamily.
STE Ser/Thr protein kinase family.
MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
O43283
Gene ID:
9175
Database links:Entrez Gene: 9175 Human
Entrez Gene: 71751 Mouse
Omim: 604915 Human
SwissProt: O43283 Human
SwissProt: Q1HKZ5 Mouse
Unigene: 656069 Human
Unigene: 420837 Mouse
Unigene: 438136 Mouse
Product Picture Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-MAP3K13/LZK (SL18663R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kD
Observed band size: 108 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP3K13) Polyclonal Antibody, Unconjugated (SL18663R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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