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Product Name MAP1D Chinese Name 蛋氨酸氨肽酶1D抗体 Alias AMP1D_HUMAN; CDS of metAP 3 within PCR fragment; M ethionine aminopeptidase 1D, mitochondrial precursor; MAP 1D; MAP1D; MetAP 1D; Metap1l; Methionine aminopeptidase 1D; Methionine aminopeptidase 1D, mitochondrial; Methionyl aminopeptidase type 1D (mitochondrial); Methionyl aminopeptidase type 1D, mitochondrial; mitochondrial; Peptidase M 1D. Research Area Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Dog, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 35kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MAP1D: 251-335/335 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]
Function:
Removes the amino-terminal methionine from nascent proteins (By similarity). May play a role in colon tumorigenesis.
Subcellular Location:
Mitochondrion.
Tissue Specificity:
Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level).
Similarity:
Belongs to the peptidase M24A family.
SWISS:
Q6UB28
Gene ID:
254042
Database links:Entrez Gene: 254042 Human
Entrez Gene: 66559 Mouse
Omim: 610267 Human
SwissProt: Q6UB28 Human
SwissProt: Q9CPW9 Mouse
Unigene: 298250 Human
Unigene: 152796 Mouse
Unigene: 24026 Rat
Product Picture Sample:
SW480(Human) Cell Lysate at 30 ug
Primary: Anti-MAP1D (SL18659R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (SL18659R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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