Product Name	MAK3/C14orf35
Chinese Name	14号染色体开放阅框35抗体
Alias	C14orf35; Chromosome 14 open reading frame 35; FLJ35355; MAK 3; Mak3p; MGC141884; MGC141885; N acetyltransferase 12 (GCN5 related putative); N acetyltransferase 12; N acetyltransferase MAK3 homolog; NAT12; Putative N acetyltransferase.
Research Area	Tumour  Cell biology  Signal transduction  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Pig, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MAK3/C14orf35: 251-350/362
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	MAK3 belongs to the acetyltransferase family, MAK3 subfamily. It contains one N-acetyltransferase domain. It is a probable N-acetyltransferase. Function: Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. Necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate. Subunit: Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of NAA35, NAA38 and NAA30. Subcellular Location: Cytoplasmic Similarity: Belongs to the acetyltransferase family. MAK3 subfamily. Contains 1 N-acetyltransferase domain. SWISS: Q147X3 Gene ID: 122830 Database links: Entrez Gene: 122830 Human Entrez Gene: 70646 Mouse Entrez Gene: 288108 Rat SwissProt: Q147X3 Human SwissProt: Q8CES0 Mouse Unigene: 165465 Human Unigene: 275688 Mouse Unigene: 15205 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C14orf35) Polyclonal Antibody, Unconjugated (SL18638R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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