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Product Name LIPC Chinese Name 肝脂酶抗体 Alias HDLCQ12; Hepatic lipase; Hepatic triacylglycerol lipase; HL; HTGL; Lipase member C; lipC; LIPC_HUMAN; LIPH. Research Area Cardiovascular Cell biology immunology The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 53kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human LIPC: 301-400/499 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]
Function:
Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Subcellular Location:
Secreted.
DISEASE:
Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency) [MIM:151670].
Similarity:
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain.
SWISS:
P11150
Gene ID:
3990
Database links:Entrez Gene: 3990 Human
Omim: 151670 Human
SwissProt: P11150 Human
Unigene: 654472 Human
Product Picture Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Rat Kidney tissue lysates
Primary: Anti-LIPC (SL18288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LIPC Polyclonal Antibody, Unconjugated(SL18288R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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