Product Name	LINS
Chinese Name	LINS蛋白抗体
Alias	LINES_HUMAN; LINS; Protein Lines homolog 1; Protein Lines homolog; Wnt-signaling molecule Lines homolog 1.
Research Area	Cell biology  immunology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	86kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LINS: 121-220/757
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Drosophila segment polarity gene lin encodes a protein, lines, which plays important roles in development of the epidermis and hindgut. This gene encodes a protein containing a lines-like domain. This gene is located on chromosome 15 and clustered with the gene encoding ankyrin repeat and SOCS box-containing protein 7. [provided by RefSeq, Sep 2010] Tissue Specificity: Expresssed in adult testis, prostate, prostate, spleen, thymus, skeletal muscle, fetal kidney and brain. Similarity: Belongs to the protein lines family. SWISS: Q8NG48 Gene ID: 55180 Database links: Entrez Gene: 55180 Human Omim: 610350 Human SwissProt: Q8NG48 Human Unigene: 105633 Human
Product Picture	Sample: U87MG(Human) Cell Lysate at 40 ug Primary: Anti-LINS (SL18286R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD Sample: U251(Human) Cell Lysate at 40 ug Primary: Anti-LINS (SL18286R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LINS) Polyclonal Antibody, Unconjugated (SL18286R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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