Product Name	HTRA4
Chinese Name	丝氨酸肽酶4抗体
Alias	FLJ90724; High temperature requirement protean A4; High-temperature requirement factor A4; HTRA 4; HtrA serine peptidase 4; HTRA4; HTRA4_HUMAN; Probable serine protease HTRA4; Serine protease HTRA4.
Research Area	Cell biology  Signal transduction  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	49kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human HTRA4: 251-350/476
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies on other related family members, this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis, and aging. [provided by RefSeq, Oct 2008] Function: Serine protease. Subcellular Location: Secreted. Similarity: Belongs to the peptidase S1B family. Contains 1 IGFBP N-terminal domain. Contains 1 Kazal-like domain. Contains 1 PDZ (DHR) domain. SWISS: P83105 Gene ID: 203100 Database links: Entrez Gene: 203100 Human Entrez Gene: 330723 Mouse Omim: 610700 Human SwissProt: P83105 Human SwissProt: A2RT60 Mouse Unigene: 661014 Human Unigene: 334452 Mouse
Product Picture	Protein: DU145(human) lyates at 30ug; Primary: Rabbit Anti-HTRA4(SL18101) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:49 kD Observed band size:50 kD Sample: PC-3 Cell (Human) Lysate at 30 ug Primary: Anti- HTRA4 (SL18101R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-HTRA4 (SL18101R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 50 kDa Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HTRA4 Polyclonal Antibody, Unconjugated(SL18101R) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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