Product Name	HSPA4L
Chinese Name	热休克蛋白70蛋白4样蛋白抗体
Alias	94kDa; AI461691; APG 1; HS74L_HUMAN; Heat shock 70kDa protein 4 like; MGC117843; MGC187594; Osmotic stress protein 94; Osp94; OTTMUSP00000027739.
Research Area	Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	94kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human HSPA4L: 101-200/839
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, which include the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The HSP 110 family (also known as the HSP 105 family) is composed of HSP 105, Apg-1 and Apg-2. Apg-1, also known as HSPA4L (heat shock 70 kDa protein 4-like) or Osp94 (osmotic stress protein 94), is an 839 amino acid protein that possesses chaperone activity in vitro, where it inhibits aggregation of citrate synthase. A homodimer, Apg-1 subcellularly localizes to cytoplasm and nucleus, and may translocate to nucleus after heat shock. Function: The HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis. Subunit: Homodimer (By similarity). Subcellular Location: Cytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock Similarity: Belongs to the heat shock protein 70 family. SWISS: O95757 Gene ID: 22824 Database links: Entrez Gene: 22824 Human Entrez Gene: 18415 Mouse Entrez Gene: 294993 Rat SwissProt: O95757 Human SwissProt: P48722 Mouse SwissProt: P83581 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSPA4L) Polyclonal Antibody, Unconjugated (SL18082R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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