Rabbit Anti-GPR78 antibody
GPCR GPR78; G protein-coupled receptor 78; G protein coupled receptor GPR78; G-protein coupled receptor 78; GPR78; GPR78_HUMAN; PRO19818; UNQ5925.
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Product Name GPR78 Chinese Name G protein-coupled receptor78抗体 Alias GPCR GPR78; G protein-coupled receptor 78; G protein coupled receptor GPR78; G-protein coupled receptor 78; GPR78; GPR78_HUMAN; PRO19818; UNQ5925. Research Area immunology Signal transduction Growth factors and hormones Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 39kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GPR78: 201-300/363 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
Function:
Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
High level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
Q96P69
Gene ID:
27201
Database links:Entrez Gene: 27201 Human
Omim: 606921 Human
SwissProt: Q96P69 Human
Unigene: 350588 Human
Unigene: 740355 Human
Product Picture 
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-GPR78(SL1807R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Blank control (blue line): U937 (blue).
Primary Antibody (green line): Rabbit Anti-GPR7 antibody (SL1807R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: 293T Cells(blue).
Primary Antibody: Rabbit Anti-GPR78/AF488 Conjugated antibody (SL1807R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (SL1807R-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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