Product Name	MRPL14
Chinese Name	Mitochondrion核糖体蛋白L14抗体
Alias	1110006I11Rik; 39S ribosomal protein L14; 39S ribosomal protein L14 mitochondrial; 39S ribosomal protein L32; AI846816; L14mt; L32mt; mitochondrial; MRP L14; MRP L32; MRP-L14; MRP-L32; mrpl14; RM14_HUMAN.
Research Area	Cell biology  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Cow, Horse, Rabbit, Sheep, )
Applications	IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	13kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MRPL14: 61-145/145
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3. [provided by RefSeq, Jul 2008] Function: Belongs to the ribosomal protein L14P family. Subunit: art of the mitochondrial ribosome large subunit (39S) (By similarity); associates with MALSU1. Subcellular Location: Mitochondrion. Similarity: Belongs to the ribosomal protein L14P family. SWISS: Q6P1L8 Gene ID: 64928 Database links: Entrez Gene: 614579 Cow Entrez Gene: 64928 Human Entrez Gene: 68463 Mouse Entrez Gene: 301250 Rat Omim: 611827 Human SwissProt: Q1JQ99 Cow SwissProt: Q6P1L8 Human SwissProt: Q9D1I6 Mouse SwissProt: Q7M0E7 Rat Unigene: 311190 Human Unigene: 379158 Mouse Unigene: 35669 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRPL14) Polyclonal Antibody, Unconjugated (SL17770R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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