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Product Name OTUD5/DUBA Chinese Name 去Ubiquitin酶A抗体 Alias Deubiquinating enzyme A; Deubiquitinating enzyme A; DKFZp761A052; DUBA; MGC104871; OTTHUMP00000025821; OTU domain containing 5; OTU domain containing protein 5; OTU domain-containing protein 5; OTUD 5; otud5; OTUD5_HUMAN. Research Area Cell biology transcriptional regulatory factor Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 61kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human OTUD5/DUBA : 501-571/571 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]
Function:
Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.
Subunit:
Interacts with TRAF3.
Tissue Specificity:
Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.
Post-translational modifications:
Phosphorylation at Ser-177 is required for deubiquitinating activity.
Similarity:
Belongs to the peptidase C85 family.
Contains 1 OTU domain.
SWISS:
Q96G74
Gene ID:
55593
Database links:Entrez Gene: 55593 Human
Entrez Gene: 54644 Mouse
Omim: 300713 Human
SwissProt: Q96G74 Human
SwissProt: Q3U2S4 Mouse
Unigene: 496098 Human
Unigene: 142827 Mouse
Unigene: 97794 Rat
Product Picture Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OTUD5 Polyclonal Antibody, Unconjugated(SL17566R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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