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Product Name Shroom 3 Chinese Name Shroom 3蛋白抗体 Alias APXL3; hShrmL; MSTP013; Protein Shroom3; Shrm; Shrm3; SHRM3_HUMAN; ShrmL; Shroom family member 3; Shroom-related protein; shroom3. Research Area Cell biology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 217kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Shroom 3: 101-200/1996 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a PDZ-domain-containing protein that belongs to a family of Shroom-related proteins. This protein may be involved in regulating cell shape in certain tissues. A similar protein in mice is required for proper neurulation. [provided by RefSeq, Jan 2011]
Function:
Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.
Subunit:
Interacts with F-actin.
Subcellular Location:
Cell junction; adherens junction. Cytoplasm > cytoskeleton. Colocalizes with F-actin in stress fibers and adherens junctions.
Similarity:
Belongs to the shroom family.
Contains 1 ASD1 domain.
Contains 1 ASD2 domain.
Contains 1 PDZ (DHR) domain.
SWISS:
Q8TF72
Gene ID:
57619
Database links:Entrez Gene: 57619 Human
Omim: 604570 Human
SwissProt: Q8TF72 Human
Unigene: 702168 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Shroom family member 3; Shroom 3) Polyclonal Antibody, Unconjugated (SL17474R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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