Product Name	TRIM45
Chinese Name	TRIM45蛋白抗体
Alias	FLJ13181; RING finger protein 99; RNF99; TRI45_HUMAN; TRIM45; Tripartite motif containing 45; Tripartite motif containing protein 45; Tripartite motif-containing protein 45.
Research Area	Cell biology  transcriptional regulatory factor  Zinc finger protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	64kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TRIM45: 101-200/580
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] Function: May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed in skeletal muscle, brain, heart and pancreas. Similarity: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 filamin repeat. Contains 1 RING-type zinc finger. SWISS: Q9H8W5 Gene ID: 80263 Database links: Entrez Gene: 80263 Human Entrez Gene: 229644 Mouse Entrez Gene: 295323 Rat Omim: 609318 Human SwissProt: Q9H8W5 Human SwissProt: Q6PFY8 Mouse Unigene: 301526 Human Unigene: 189351 Mouse Unigene: 102590 Rat
Product Picture	Sample: Siha Cell (Human) Lysate at 30 ug Primary: Anti-TRIM45 (SL16739R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64kD Observed band size: 64kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM45) Polyclonal Antibody, Unconjugated (SL16739R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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