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Product Name IME1 Chinese Name 减数分裂诱导蛋白IME1抗体 Alias Meiosis inducing protein; IME1_YEAST; YJR094C; Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). 酿酒酵母; Research Area Cell biology transcriptional regulatory factor Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae IME1: 301-360/360 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Meiosis-inducing protein (IME1) is a master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p. IME1 is required for sporulation and for early sporulation-specific genes expression. It is a positive regulator of SME1/IME2 expression.
Function:
Transcription factor required for sporulation and for early sporulation-specific genes expression. Positive regulator of SME1/IME2 expression. Directly activates expression of SLZ1 during meiosis.
Subunit:
Interacts with UME6.
Subcellular Location:
Nuclear
SWISS:
P21190
Gene ID:
N/A
Database links:Entrez Gene: 853556 Saccharomyces cerevisiae
SwissProt: P21190 Saccharomyces cerevisiae
Product Picture Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IME1 Polyclonal Antibody, Unconjugated(SL16619R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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