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Product Name TNFAIP8L3 Chinese Name Tumour坏死因子诱导蛋白8样蛋白3/TNFα-IP 8L3抗体 Alias 9930029P06Rik; FLJ41287; Gm517; MGC141255; RGD1311533; TNF alpha-induced protein 8-like protein 3; TNFAIP8-like protein 3; tnfaip8l3; TP8L3_HUMAN; Tumor necrosis factor alpha-induced protein 8-like protein 3. literatures Specific References (1) | SL16609R has been referenced in 1 publications.[IF=3.743] Gao JF et al. Effects of the long and short isoforms of TIPE3 on the growth and metastasis of gastric cancer. Biomedicine & Pharmacotherapy, 2020 124, 109853. IHC-P ; Human&Mouse.Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 33kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TNFAIP8L3: 81-180/292 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TNFAIP8L3 Polyclonal Antibody, Unconjugated(SL16609R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP8L3) Polyclonal Antibody, Unconjugated (SL16609R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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