Product Name	Transmembrane protein 30A
Chinese Name	Transmembrane protein30A抗体
Alias	C6orf67; CDC50A; CC50A_HUMAN; Cell cycle control protein 50A; FLJ10856; TMEM30A.
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Pig, Cow, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	41kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Transmembrane protein 30A: 251-350/361
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cdc50A is a 361 amino acid multi-pass membrane protein that is expressed in placenta, brain and embryonic stem cells and may play a role in cell division. The gene encoding Cdc50A maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6 Subcellular Location: Membrane; Multi-pass membrane protein Similarity: Belongs to the CDC50/LEM3 family. SWISS: Q9NV96 Gene ID: 55754 Database links: Entrez Gene: 55754 Human Entrez Gene: 69981 Mouse Entrez Gene: 300857 Rat Entrez Gene: 337397 Zebrafish Omim: 611028 Human SwissProt: E2R2Z8 Dog SwissProt: Q9NV96 Human SwissProt: Q8VEK0 Mouse SwissProt: D3ZYA3 Rat SwissProt: Q6TNV5 Zebrafish
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Transmembrane protein 30A Polyclonal Antibody, Unconjugated(SL16576R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Transmembrane protein 30A) Polyclonal Antibody, Unconjugated (SL16576R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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