Product Name	FGD5
Chinese Name	FGD5蛋白抗体
Alias	FYVE, RhoGEF and PH domain containing protein 5; ZFYVE23; FGD5_HUMAN; Zinc finger FYVE domain containing protein 23.
Research Area	Cell biology  immunology  Signal transduction  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Dog, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	160kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FGD5: 1301-1462/1462
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	FGD5 is a 1,462 amino acid cytosolic protein that contains a DH domain, a FYVE-type zinc finger and two PH domains. FGD family members encode guanine nucleotide exchange factors that specifically activate the Rho GTPase Cdc42. All FGD proteins contain equivalent signaling domains and a conserved structural organization, which strongly suggests that these signaling domains form a canonical core structure for members of the FGD family of RhoGEF proteins. These proteins also control essential signals required during embryonic development. Function: FGD5 may activate CDC42, a member of the Ras like family of Rho and Rac proteins, by exchanging bound GDP for free GTP. It may also play a role in regulating the actin cytoskeleton and cell shape. FGD5 contains one DH (DBL-homology) domain one FYVE type zinc finger and two PH domains. There are two isoforms. Subcellular Location: Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Endoplasmic reticulum. Golgi apparatus. Early endosome. Note=In peripheral membrane ruffles, colocolizes with F-actin. In confluent HUVECs, detected at cell-cell-contact sites where it colocalizes with vascular endothelial cadherin/CDH5. Tissue Specificity: Expressed in endothelial cells (at protein level). Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 FYVE-type zinc finger. Contains 2 PH domains. SWISS: Q6ZNL6 Gene ID: 152273 Database links: Entrez Gene: 152273 Human Entrez Gene: 232237 Mouse Entrez Gene: 362402 Rat Omim: 614788 Human SwissProt: Q6ZNL6 Human SwissProt: Q80UZ0 Mouse Unigene: 412406 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGD5) Polyclonal Antibody, Unconjugated (SL16081R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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