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Product Name FGD1 Chinese Name FGD1蛋白抗体 Alias AAS; Faciogenital dysplasia 1 protein; FGD1; FGD1_HUMAN; FGDY; FYVE RhoGEF and PH domain containing protein 1; FYVE, RhoGEF and PH domain-containing protein 1; Rho/Rac GEF; Rho/Rac guanine nucleotide exchange factor FGD1; ZFYVE3; Zinc finger FYVE domain containing protein 3; Zinc finger FYVE domain-containing protein 3. Research Area Cell biology immunology Developmental biology Signal transduction G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 107kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FGD1: 601-700/961 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Activates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
Function:
Activates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
Subcellular Location:
Cytoplasm. Cell projection > lamellipodium. Cell projection > ruffle. Cytoplasm > cytoskeleton. Associated with membrane ruffles and lamellipodia.
Tissue Specificity:
Expressed in fetal heart, brain, lung, kidney and placenta. Less expressed in liver; adult heart, brain, lung, pancreas and skeletal muscle.
DISEASE:
Defects in FGD1 are the cause of Aarskog-Scott syndrome (AAS) [MIM:305400]. This faciogenital dysplasia is a rare multisystemic disorder characterized by disproportionately short stature, and by facial, skeletal, and urogenital anomalies.
Note=Defects in FGD1 are found in a pateint with non-syndromal X-linked mental retardation.
Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 FYVE-type zinc finger.
Contains 2 PH domains.
SWISS:
P98174
Gene ID:
2245
Database links:Entrez Gene: 2245 Human
Omim: 305400 Human
SwissProt: P98174 Human
Unigene: 631767 Human
Unigene: 709201 Human
Product Picture Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-FGD1 (SL16077R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 107 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGD1) Polyclonal Antibody, Unconjugated (SL16077R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGD1) Polyclonal Antibody, Unconjugated (SL16077R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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