TEL: +86 571 56623320 EMAIL: [email protected]
Product Name CYP1A1 Chinese Name 细胞色素P450 1A1抗体 Alias Cytochrome p450 1A1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; AHH; AHRR; Aryl hydrocarbon hydroxylase; CYP 1; CYP1A1; CYPIA1; Cytochrome P1-450; Flavoprotein-linked monooxygenase; Microsomal monooxygenase; P1 450; P450 C; P450 form 6; P450 P1; Xenobiotic monooxygenase. literatures Specific References (4) | SL1606R has been referenced in 4 publications.[IF=4.932] Wenjuan Xun. et al. Resveratrol protects intestinal integrity, alleviates intestinal inflammation and oxidative stress by modulating AhR/Nrf2 pathways in weaned piglets challenged with diquat. Int Immunopharmacol. 2021 Oct;99:107989 WB ; Pig.[IF=3.269] Jiasui Wang. et al. Aryl hydrocarbon receptor is a prognostic biomarker and is correlated with immune responses in cervical cancer. 2021 Nov 16 IHC ; Human.[IF=3.12] Yan R et al. PM2. 5 exposure induces age-dependent hepatic lipid metabolism disorder in female mice. journal of environmental science.2019. WB ; Mouse.[IF=2.34] Cedervall, Therése, Pia Monica Lind, and Lars Sävendahl. "Expression of the Aryl Hydrocarbon Receptor in Growth Plate Cartilage and the Impact of Its Local Modulation on Longitudinal Bone Growth." International Journal of Molecular Sciences 16.4 (2015): 8059-8069. IHC-P ; Human.Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 58kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CYP1A1: 361-460/512 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several cofactors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
Function:
Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.
Subcellular Location:
Cytochrome P450 1A1: Cytoplasm.
Cytochrome P450MT2A: Endoplasmic reticulum membrane. Mitochondrion membrane. Microsome membrane.
Cytochrome P450MT2B: Endoplasmic reticulum. Mitochondrion.
Tissue Specificity:
Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary.
Similarity:
Belongs to the cytochrome P450 family.
SWISS:
A9YTQ3
Gene ID:
57491
Database links:Entrez Gene: 57491 Human
Entrez Gene: 11624 Mouse
Omim: 606517 Human
SwissProt: A9YTQ3 Human
SwissProt: Q3U1U7 Mouse
Unigene: 50823 Human
Unigene: 290446 Mouse
Unigene: 145524 Rat
Synthesis and Degradation(Synthesis and Degradation)Product Picture Sample:K562 Cell (Human) Lysate at 30 ug
Primary: Anti- CYP1A1 (SL1606R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Sample:Lung (Mouse) Lysate at 40 ug
Primary: Anti- CYP1A1 (SL1606R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP1A1) Polyclonal Antibody, Unconjugated (SL1606R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode