Product Name	Cathepsin G
Chinese Name	组织蛋白酶G抗体
Alias	CTSG; Cathepsin G; CG; Cathepsin G precursor; CathepsinG; CTSG; CTSG protein; MGC23078; CATG_HUMAN.
Research Area	Cell biology  immunology  Synthesis and Degradation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	28kDa
Cellular localization	The nucleus The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CTSG: 41-120/255
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cathepsin G is a serine protease, it consisting of a single polypeptide with a molecular weight of 30 kDa as determined by SDS-PAGE. Its substrates are collagen and proteoglycans. Poor regulation of Cathepsin G levels has been associated with various connective tissue disorders, for example pulmonary emphysema and cystic fibrosis. Function: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. Subcellular Location: Cell surface. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: P08311 Gene ID: 1511 Database links: Entrez Gene: 1511 Human Omim: 116830 Human SwissProt: P08311 Human Unigene: 421724 Human
Product Picture	Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin G Polyclonal Antibody, Unconjugated(SL1598R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cathepsin G Antibody(SL1598R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).

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