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Product Name F-Actin Chinese Name 纤维状肌动蛋白抗体 Alias f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1. literatures Specific References (31) | SL1571R has been referenced in 31 publications.Research Area immunology Cytoskeleton Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Pig, Cow, Sheep, ) Applications WB=1:1000-5000 ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=3μg /test (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen Synthetic MAP peptide derived from human beta-Actin: 1-50/374 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins, found in muscletissues, are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin.
SWISS:
P60709
Gene ID:
60
Database links:Entrez Gene: 60 Human
Entrez Gene: 11461 Mouse
Entrez Gene: 100009272 Rabbit
Omim: 102630 Human
SwissProt: P60709 Human
SwissProt: P60710 Mouse
SwissProt: P29751 Rabbit
Unigene: 520640 Human
Unigene: 708120 Human
Unigene: 727576 Human
Unigene: 328431 Mouse
Unigene: 391967 Mouse
Unigene: 94978 Rat
纤维状肌动蛋白F-Actin主要存在于横纹肌原肌纤维细丝及平滑肌中,是肌肉运动和细胞运动的重要蛋白。Product Picture Sample: Lane1:Hela (Human) Whole Cell Lysate at 30 ug
Lane2:Jurkat (Human) Whole Cell Lysate at 30 ug
Lane3:293T (Human) Whole Cell Lysate at 30 ug
Lane4:Hepg2 (Human) Whole Cell Lysate at 30 ug
Lane5:A431 (Human) Whole Cell Lysate at 30 ug
Lane6:A549 (Human) Whole Cell Lysate at 30 ug
Lane7:H9C2 (Rat) Whole Cell Lysate at 30 ug
Lane8:Testis (Mouse) Tissue Lysate at 30 ug
Primary: Anti-F-Actin(SL1571R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (SL1571R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti- F-Actin antibody (SL1571R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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