Product Name	Annexin A1
Chinese Name	膜粘连蛋白A1抗体
Alias	Annexin I; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; Calpactin II; CalpactinII; Chromobindin 9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; LPC 1; LPC1; p35; Phospholipase A2 inhibitory protein; ANXA1_HUMAN.
literatures	Specific References  (2)     |     SL1562R has been referenced in 2 publications. [IF=3.35] Li, Yangguang, et al. "Glycoproteomic analysis of tissues from patients with colon cancer using lectin microarrays and nanoLC-MS/MS." Mol. BioSyst. (2013).  WB ;  Human.   PubMed:23567825 [IF=2.46] Wang, Zhong-Chao, et al. "2D-DIGE proteomic analysis of changes in estrogen/progesterone-induced rat breast hyperplasia upon treatment with the Mongolian remedy RuXian-I." Molecules 16.4 (2011): 3048-3065.  Rat.   PubMed:21478820
Research Area	Tumour
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Annexin A1: 281-346/346
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014] Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. Subunit: Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF. Subcellular Location: Nucleus. Cytoplasm. Cell projection, cilium. Basolateral cell membrane. Note=Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. Post-translational modifications: Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P04083 Gene ID: 301 Database links: Entrez Gene: 301 Human Entrez Gene: 16952 Mouse Entrez Gene: 25380 Rat SwissProt: P04083 Human SwissProt: P10107 Mouse SwissProt: P07150 Rat
Product Picture	Sample: Lane1: Kidney(Rat) Lysate at 30 ug Lane2: Brain(Rat) Lysate at 30 ug Primary: Anti-Annexin A1 (SL1562R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 39kD Observed band size : 40kD Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min; Incubation: Anti-Annexin A1 Polyclonal Antibody, Unconjugated(SL1562R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Annexin A1) polyclonal Antibody, Unconjugated (SL1562R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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