Product Name	Alkaline Phosphatase, Tissue Non-Specific isozyme
Chinese Name	碱性磷酸酶(组织非特异性)抗体
Alias	AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP.  碱性磷酸酶，组织非特异性同工酶; 组织非特异性碱性磷酸酶; 碱性磷酸酶,组织非特异性;
literatures	Specific References  (4)     |     SL1535R has been referenced in 4 publications. [IF=5.595] Cao D et al. Hematopoietic stem cells and lineage cells undergo dynamic alterations under microgravity and recovery conditions.FASEB J. 2019 Feb 27:fj201802421RR.  FCM ;  Mouse.   PubMed:30811956 [IF=4.545] Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235.  IHC/IF ;  Mouse.   PubMed:32454289 [IF=2.147] Huijuan Shen. et al. microRNA-146a mediates distraction osteogenesis via bone mesenchymal stem cell inflammatory response. ACTA HISTOCHEM. 2022 Aug;124:151913  WB ;  Dog.   PubMed:35759812 [IF=1.931] Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034  WB ;  Human.   PubMed:33472098
Research Area	Cell biology  immunology  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	56kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Alkaline Phosphatase, Tissue Non-Specific isozyme: 52-150/524
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Function: This isozyme may play a role in skeletal mineralization. Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Post-translational modifications: Glycosylated. DISEASE: Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510]. Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500]. Similarity: Belongs to the alkaline phosphatase family. SWISS: P05186 Gene ID: 249 Database links: Entrez Gene: 249 Human Entrez Gene: 11647 Mouse Entrez Gene: 25586 Rat Omim: 171760 Human SwissProt: P05186 Human SwissProt: P09242 Mouse SwissProt: P08289 Rat Unigene: 75431 Human Unigene: 288186 Mouse Unigene: 82764 Rat ALP广泛分布于人体肝脏、骨骼、肠、肾和胎盘等组织,孕妇、骨折愈合期、骨软化症。佝偻病、骨细胞癌、骨质疏松、肝脓肿、肝结核、肝硬变、白血病、甲状腺机能亢进时，血清碱性磷酸酶亦可升高.
Product Picture	Blank control (blue line): Hep G2(blue). Primary Antibody (green line): Rabbit Anti-ALPL antibody (SL1535R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 6

  6Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• A

  ADelete

• 
• A

  ADelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout