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Product Name ERp57 Chinese Name 内质网蛋白57抗体 Alias ERp57/ERp60; 58 kDa glucose regulated protein; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER 60; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER p57; ER protein 57; ER protein 60; ERp 57; ERp57; ERp60; ERp61; Glucose Regulated Protein 58 Kd; GRP 57; GRP 58; GRP57; GRP58; HsT17083; P58; PDIA 3; PDIA3; PDIA3_HUMAN; Phospholipase C alpha; PI PLC; Protein disulfide isomerase A3; Protein disulfide isomerase family A member 3; Protein disulfide-isomerase A3. literatures Specific References (1) | SL14629R has been referenced in 1 publications.[IF=3.548] Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. WB ; Mouse.Research Area Cell biology Transporter glycoprotein Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 54kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ERp57: 351-450/505 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity:
Detected in the flagellum and head region of spermatozoa (at protein level).
Similarity:
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
SWISS:
P30101
Gene ID:
2923
Database links:Entrez Gene: 2923 Human
Entrez Gene: 14827 Mouse
Entrez Gene: 100328685 Rabbit
Omim: 602046 Human
SwissProt: P30101 Human
SwissProt: P27773 Mouse
Unigene: 591095 Human
Unigene: 263177 Mouse
Unigene: 11527 Rat
Product Picture Sample:
embryo (Mouse) Lysate at 40 ug
Primary: Anti-ERp57 (SL14629R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERp57) Polyclonal Antibody, Unconjugated (SL14629R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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