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Product Name DDX55 Chinese Name ATP依赖RNA解旋酶DDX55抗体 Alias ATP dependent RNA helicase DDX55; ATP-dependent RNA helicase DDX55; DDX 55; ddx55; DDX55_HUMAN; DEAD box protein 55; FLJ16577; KIAA1595; MGC33209. Research Area Developmental biology Binding protein Cell differentiation Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 69kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DDX55: 451-550/600 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been confirmed. [provided by RefSeq, Jul 2008]
Function:
Probable ATP-binding RNA helicase.
Similarity:
Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
SWISS:
Q8NHQ9
Gene ID:
57696
Database links:Entrez Gene: 57696 Human
Entrez Gene: 67848 Mouse
Entrez Gene: 286745 Zebrafish
SwissProt: Q8NHQ9 Human
SwissProt: Q6ZPL9 Mouse
SwissProt: Q8JHJ2 Zebrafish
Unigene: 286173 Human
Unigene: 281601 Mouse
Unigene: 16883 Zebrafish
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX55) Polyclonal Antibody, Unconjugated (SL14336R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX55) Polyclonal Antibody, Unconjugated (SL14336R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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