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Product Name CYFIP2 Chinese Name 细胞质FMR1相关蛋白2抗体 Alias CYFP2; cytoplasmic FMR1 interacting protein 2; KIAA1168; p53 inducible protein; p53-inducible protein 121; PIR121; CYFP2_HUMAN. Research Area Cell biology Developmental biology Neurobiology Signal transduction t-lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 146kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CYFIP2: 251-350/1278 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CYFIP2 is a 1,278 amino acid protein encoded by the human gene CYFIP2. CYFIP2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. It interacts with FMR1, FXR1 and FXR2 and is a component of the WAVE1 complex composed of Abi-2, CYFIP2, C3orf10/HSPC300, NAP125 and WASF1/WAVE1. Upon binding to activated Rac 1, CYFIP2 causes the complex to dissociate, releasing activated WASF1. The CYFIP2 promoter contains a p53-responsive element that confers p53 binding as well as transcriptional activation of a heterologous reporter. Induced expression of CYFIP2 is sufficient for caspase activation and cellular apoptosis, reminiscent of p53 activation.
Function:
CYFIP2 is involved in T cell adhesion and p53 dependent induction of apoptosis. It does not bind RNA but is up regulated significantly in CD4+ T lymphocytes from patients with multiple sclerosis. There are 2 isoforms produced by alternative splicing.
Subcellular Location:
Cytoplasm perinuclear region. Cell junction, synapse, synaptosome. Treatment with leptomycin-B triggers translocation to the nucleus.
Similarity:
Belongs to the CYFIP family.
SWISS:
Q96F07
Gene ID:
26999
Database links:Entrez Gene: 26999 Human
Entrez Gene: 76884 Mouse
Omim: 606323 Human
SwissProt: Q96F07 Human
SwissProt: Q5SQX6 Mouse
Unigene: 910449 Human
Unigene: 293961 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYFIP2) Polyclonal Antibody, Unconjugated (SL14140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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