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Product Name Cyclin G Chinese Name 周期素G抗体 Alias CCNG 1; CCNG; CCNG1; CYCG1; Cyclin G; CCNG1_HUMAN; Cyclin-G; Cyclin G1; CyclinG1; Cyclin-G1. literatures Specific References (1) | SL1389R has been referenced in 1 publications.[IF=0.352] Xu, Qian, et al. "CYCLIN G1 INHIBITS THE PROLIFERATION OF MOUSE ENDOMETRIAL STROMAL CELL IN DECIDUALIZATION."Arch Biol Sci. 2017;69(1):71-81. IF(ICC) ; Mouse.Research Area Cell biology Chromatin and nuclear signals Cyclin Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 34kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Cyclin G: 181-295/295 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.
Function:
May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity).
Subcellular Location:
Nucleus. DNA replication foci after DNA damage.
Tissue Specificity:
High levels in skeletal muscle, ovary, kidney and colon.
Similarity:
Belongs to the cyclin family. Cyclin G subfamily.
SWISS:
P51959
Gene ID:
900
Database links:Entrez Gene: 900 Human
Omim: 601578 Human
SwissProt: P51959 Human
Unigene: 79101 Human
Cyclin G的生物功能还没有完全清楚,他是Cyclin家族中近来发现的新成员,也是已知的唯一受到Tumour抑制基因p53调控的Cyclin。
近年来经研究发现,Cyclin G可能有促进细胞增长的作用,在细胞增殖和神经再生中的可能起到一定的作用。Product Picture Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (SL1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (SL1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (SL1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin G Polyclonal Antibody, Unconjugated(SL1389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (SL1389R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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