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Product Name APG4B Chinese Name 自噬相关蛋白4B抗体 Alias APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); AUT like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353br. Research Area Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 44kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human APG4B: 85-200/392 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Function:
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Subcellular Location:
Cytoplasm (Probable).
Tissue Specificity:
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Similarity:
Belongs to the peptidase C54 family.
SWISS:
Q9Y4P1
Gene ID:
23192
Database links:Entrez Gene: 23192 Human
Entrez Gene: 66615 Mouse
Omim: 611338 Human
SwissProt: Q9Y4P1 Human
SwissProt: Q8BGE6 Mouse
Unigene: 283610 Human
Unigene: 29087 Mouse
Unigene: 163086 Rat
Autophagy(Autophagy)是一种非凋亡性的程序性细胞死亡即自噬性程序性细胞死亡,进化过程中高度保守,清除降解细胞内受损伤的细胞结构、衰老的细胞器、以及不再需要的生物大分子等,在细胞的生长、发育和疾病发生中起着重要的作用。Autophagy是一个高效调控过程,特异性激酶和鸟嘌呤核苷酸磷酸酶(GTPases)在调节自噬通道活性中起着关键作用.Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B) Polyclonal Antibody, Unconjugated (SL1384R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APG4B/AUTL1 Polyclonal Antibody, Unconjugated(SL1384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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