Product Name	SNAI2
Chinese Name	锌指转录因子Slug抗体
Alias	SLUG; Neural crest transcription factor Slug; Protein snail homolog 2; Slug (chicken homolog) zinc finger protein; Slug homolog zinc finger protein; Slug zinc finger protein; SLUGH 1; SLUGH; SLUGH1; SNAI 2; SNAI-2; SNAI2_HUMAN; Snail 2; Snail homolog 2; Snail2; Snail-2; WS 2D; WS2D; Zinc finger protein SLUG; Zinc finger protein SNAI2.
literatures	Specific References  (7)     |     SL1382R has been referenced in 7 publications. [IF=5.02] Bai, J. W., et al. "The zinc-finger transcriptional factor Slug transcriptionally downregulates ERα by recruiting lysine-specific demethylase 1 in human breast cancer." Oncogenesis 6.5 (2017): e330.  IHC-P ;  Human.   PubMed:28481366 [IF=4.254] Zhou K et al. RAC1-GTP promotes epithelial-mesenchymal transition and invasion of colorectal cancer by activation of STAT3.Lab Invest. 2018 Aug;98(8):989-998.  IHC ;  Human.   PubMed:29884911 [IF=4.137] Huang T et al. Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer. Front Oncol. 2020 Jan 29;10:19.  IHC-P ;  Human.   PubMed:32064233 [IF=3.152] Li Z et al. Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Exp Eye Res. 2019 Apr 24;184:227-233.  IHF、WB ;  Rabbit、Human.   PubMed:31028751 [IF=3.06] Jin, Meiyuan. et al. Role of ARID1A in the Regulation of Human Trophoblast Migration and Invasion. 2021 Jul 13  WB ;  Human.   PubMed:34255312 [IF=3.02] Kong, Lingxin, et al. "Overexpression of SDF-1 activates the NF-κB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells." International Journal of Oncology.  other ;  Human.   PubMed:26782945 [IF=2.31] Liu, Baolin, et al. "Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial–mesenchymal transition via NF-κB activation." OncoTargets and Therapy 9 (2016): 1011-1021.  WB ;  Human.   PubMed:27013894
Research Area	Tumour  immunology  Neurobiology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	30kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Slug: 5-120/268
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]. Function: Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occluding (OCLN) and subsequent oncogenic transformation of epithelial cells. Subunit: Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Subcellular Location: Nucleus. Cytoplasm. Note=Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions. Tissue Specificity: Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level). Post-translational modifications: GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation. DISEASE: Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) . WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1 Similarity: Belongs to the snail C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. SWISS: O43623 Gene ID: 6591 Database links: Entrez Gene: 6591 Human Entrez Gene: 20583 Mouse Entrez Gene: 641345 Pig Entrez Gene: 25554 Rat Entrez Gene: 432368 Chicken Entrez Gene: 520631 Cow Omim: 602150 Human SwissProt: Q3MHQ4 Cow SwissProt: O43623 Human SwissProt: P97469 Mouse SwissProt: Q3UZ96 Mouse SwissProt: O08954 Rat Unigene: 360174 Human Unigene: 4272 Mouse Unigene: 43117 Rat transcriptional regulatory factor（Transcriptin Regulators） 锌指转录因子Slug主要用于消化系统Tumour转移方面的研究。
Product Picture	Sample: Lane1: Heart (Rat) Lysate at 30 ug Lane2: Liver (Rat) Lysate at 30 ug Primary: Anti-SNAI2/SLUG (SL1382R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 30kD Observed band size : 30kD Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human MDA-MB-231 cell lysates Primary: Anti-SNAI2 (SL1382R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 34 kDa Sample: Lane 1: Mouse Breast tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Human MDA-MB-231 cell lysates Lane 4: Human U2OS cell lysates Primary: Anti-SNAI2 (SL1382R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 34 kDa Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SNAI2 Polyclonal Antibody, Unconjugated(SL1382R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control（black line）:HepG2. Primary Antibody (green line): Rabbit Anti-SNAI2 antibody (SL1382R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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