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Product Name Claudin 19 Chinese Name 紧密连接蛋白19抗体 Alias Claudin 19; CLDN 19; CLD19_HUMAN. Research Area Cell biology Signal transduction Cell adhesion molecule Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 23kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Claudin 19: 21-120/224 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-19 is a 224 amino acid multi-pass membrane protein that belongs to the claudin family and is expressed as two isoforms due to alternative splicing events. Defects in the gene encoding claudin-19 are the cause of hypomagnesemia renal with ocular involvement (HOMGO), a renal disease characterized by hypomagnesemia, hypercalciuria and nephrocalcinosis.
Function:
CLDN19 belongs to the claudin family. It plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Subcellular Location:
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Similarity:
Belongs to the claudin family.
SWISS:
Q8N6F1
Gene ID:
149461
Database links:Entrez Gene: 149461 Human
Omim: 610036 Human
SwissProt: Q8N6F1 Human
Product Picture Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-claudin-19 Polyclonal Antibody, Unconjugated(SL13749R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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