Product Name	CLDN25
Chinese Name	紧密连接蛋白25抗体
Alias	claudin 25; CLD25_HUMAN; CLDN25; Gm16492; Gm513; Putative claudin-25.
Research Area	Cell biology  Signal transduction  Cell adhesion molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Dog, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CLDN25: 101-200/229
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Subcellular Location: Cell junction > tight junction. Cell membrane. Similarity: Belongs to the claudin family. SWISS: C9JDP6 Gene ID: 664672 Database links: Entrez Gene: 644672 Human SwissProt: C9JDP6 Human Unigene: 705360 Human
Product Picture	Sample: 293T (human)cell Lysate at 40 ug Primary: Anti- CLDN25(SL13745R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25kD Observed band size: 28 kD Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CLDN25 Polyclonal Antibody, Unconjugated(SL13745R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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