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Product Name LAD1 Chinese Name LAD1蛋白抗体 Alias LAD; Lad 1; Lad-1; Lad1; LAD1_HUMAN; LADA; Ladinin 1; Ladinin-1; Linear IgA bullous dermatosis antigen; Linear IgA disease antigen; Linear IgA disease antigen homolog; MGC10355. Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human LAD1: 331-430/517 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Anchoring filament protein which is a component of the basement membrane zone.
Subcellular Location:
Secreted > extracellular space > extracellular matrix > basement membrane.
SWISS:
O00515
Gene ID:
3898
Database links:Entrez Gene: 3898 Human
Omim: 602314 Human
SwissProt: O00515 Human
Unigene: 519035 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAD1) Polyclonal Antibody, Unconjugated (SL13708R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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