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Product Name GADD45A Chinese Name 生长抑制DNA损伤基因45抗体 Alias Growth Arrest And DNA Damage Inducible Alpha; DNA Damage-Inducible Transcript 1 Protein; DDIT-1; GADD45; DDIT1; Growth Arrest And DNA Damage-Inducible Protein GADD45 Alpha; Growth Arrest And DNA-Damage-Inducible 45 Alpha; Growth Arrest And DNA-Damage-Inducible, Alpha; DNA Damage-Inducible Transcript-1; GA45A_HUMAN literatures Specific References (6) | SL1360R has been referenced in 6 publications.[IF=10.447] Bin Yan. et al. Tumor and stroma COL8A1 secretion induces autocrine and paracrine progression signaling in pancreatic ductal adenocarcinoma. MATRIX BIOL. 2022 Nov;: IHC ; Mouse.[IF=7.419] Li Liu. et al. Nanosilver inhibits the progression of pancreatic cancer by inducing a paraptosis-like mixed type of cell death. BIOMED PHARMACOTHER. 2022 Sep;153:113511 IHC ; Chicken.[IF=4.489] Xin-Li Xiao. et al. Status epilepticus induced Gadd45b is required for augmented dentate neurogenesis. Stem Cell Res. 2020 Dec;49:102102 IF ; Mouse.[IF=4.2] Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829. WB ; Mouse.[IF=2.985] Qingfan Mo. et al. BTNL9 is frequently downregulated and inhibits proliferation and metastasis via the P53/CDC25C and P53/GADD45 pathways in breast cancer. Biochem Bioph Res Co. 2021 May;553:17 WB ; Human.[IF=0.65] Barış et al. Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement?. (2015) Turk.J.Haematol. 32:295-303 IHC ; Human, Mouse, Rat, Chicken, Pig, Cow.Research Area immunology Signal transduction Apoptosis Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GADD45: 65-165/165 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53 dependent and independent mechanisms.
Function:
In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
Subunit:
Interacts with MAPK14. Predominantly monomeric but also forms dimers and other oligomers as concentration increases. Interacts with GADD45GIP1. Interacts weakly with PCNA. Interacts with AURKA, likely to compete with dimerization.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the GADD45 family.
SWISS:
P24522
Gene ID:
1647
Database links:
Entrez Gene: 1647 Human
Entrez Gene: 13197 Mouse
Omim: 126335 Human
SwissProt: P24522 Human
SwissProt: P48316 Mouse
Unigene: 80409 Human
Unigene: 72235 Mouse
Unigene: 10250 Rat
GADD45α蛋白与Apoptosis和死亡密切相关,在某种程度上决定了细胞周期的进程。Product Picture Sample:
Lane1: Brain (Rat) Lysate at 30 ug
Lane2:Kidney (Rat) Lysate at 30 ug
Primary: Anti-GADD45 (SL1360R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 18kD
Observed band size : 18kD
We are unsure as to the identity of these extra bandsParaformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GADD45) Polyclonal Antibody, Unconjugated (SL1360R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GADD45 Polyclonal Antibody, Unconjugated(SL1360R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GADD45) Polyclonal Antibody, Unconjugated (SL1360R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GADD45) Polyclonal Antibody, Unconjugated (SL1360R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GADD45) Polyclonal Antibody, Unconjugated (SL1360R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.Blank control: A431.
Primary Antibody (green line): Rabbit Anti-GADD45 antibody (SL1360R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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