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Product Name ZAR1L Chinese Name 受精卵抑制蛋白1样蛋白抗体 Alias RP11-37E23.4; ZAR1-like protein; ZAR1L; ZAR1L_HUMAN; ZAR2; Zygote arrest 1 like. Research Area Cell biology immunology Developmental biology Signal transduction Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 36kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ZAR1L: 51-150/321 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the ZAR1 family that is predominantly expressed in oocytes and early embryos. The protein may function as an RNA regulator in early embryos.
Similarity:
Belongs to the ZAR1 family.
SWISS:
A6NP61
Gene ID:
646799
Database links:Entrez Gene: 646799 Human
SwissProt: A6NP61 Human
Unigene: 569254 Human
Product Picture Sample:A549 (Human)cell Lysate at 40 ug
Primary: Anti-ZAR1L(SL13550R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 36kD
Observed band size: 36kD
Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZAR1L) Polyclonal Antibody, Unconjugated (SL13550R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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