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Product Name GNPDA2 Chinese Name 葡萄糖6-磷酸脱氨酶2抗体 Alias GlcN6P deaminase 2; Glucosamine 6 phosphate deaminase 2; Glucosamine 6 phosphate isomerase 2; Glucosamine 6 phosphate isomerase SB52; Glucosamine-6-phosphate deaminase 2; Glucosamine-6-phosphate isomerase 2; Glucosamine-6-phosphate isomerase SB52; GNP2; GNPDA 2; Gnpda2; GNPI2_HUMAN; SB52. Research Area Tumour Cell biology Signal transduction The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 31kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GNPDA2: 101-200/276 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail During fertilization in mammals, the sperm activates the egg by causing an increase in the level of free cytoplasmic calcium concentration. This increased calcium concentration induces a characteristic series of oscillations that trigger egg activation and early embryo development. A hamster protein named oscillin is thought to be involved in this pathway. The enzyme glucosamine-6-phosphate isomerase (GNPI) or deaminase (GNPDA1) and the related protein GNPDA2 are the human homologs of hamster oscillin. GNPDA1 and GNPDA2 catalyze the conversion of GNP to fructose-6-phosphate and ammonia. Both proteins exist as homohexamers and are ubiquitously expressed with highest expression in testis, ovary and heart. Three isoforms of GNPDA2 are expressed due to alternative splicing events.
Subunit:
Homohexamer (By similarity).
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.
SWISS:
Q8TDQ7
Gene ID:
132789
Database links:Entrez Gene: 132789 Human
Entrez Gene: 67980 Mouse
Omim: 613222 Human
SwissProt: Q8TDQ7 Human
SwissProt: Q9CRC9 Mouse
Unigene: 21398 Human
Unigene: 33830 Mouse
Product Picture Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- GNPDA2 (SL13474R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GNPDA2) Polyclonal Antibody, Unconjugated (SL13474R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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