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Rabbit Anti-GSTA1 antibody
Rabbit Anti-GSTA1 antibody
Glutathione S alkyltransferase A1; Glutathione S aralkyltransferase A1; Glutathione S aryltransferase A1; Glutathione S transferase 2; Glutathione S transferase A1; Glutathione S transferase alpha 1; Glutathione S transferase Ha subunit 1; Glutathione S-t
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  • NO.:SL13398R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,Rat,(predicted: Human,Dog,Horse,Rabbit,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Product Name GSTA1
Chinese Name 谷胱甘肽S转移酶α1抗体
Alias Glutathione S alkyltransferase A1; Glutathione S aralkyltransferase A1; Glutathione S aryltransferase A1; Glutathione S transferase 2; Glutathione S transferase A1; Glutathione S transferase alpha 1; Glutathione S transferase Ha subunit 1; Glutathione S-transferase A1; GST 2; GST class alpha; GST class alpha 1; GST class alpha member 1; GST class-alpha member 1; GST epsilon; GST HA subunit 1; GST, class alpha, 1; GST-epsilon; GST2; GSTA 1; GSTA1 1; GSTA1 protein; GSTA1-1; GSTA1_HUMAN; GTH 1; GTH1; HA subunit 1; MGC131939; OTTHUMP00000016611; S (hydroxyalkyl)glutathione lyase A1.  
literatures
Specific References  (1)     |     SL13398R has been referenced in 1 publications.
[IF=1.718] Efe Saygın. et al. Glutathione S-transferase expression in benign and malignant eyelid tumors. 2021 Oct 26  IHC ;  Human.  
Research Area Tumour  Signal transduction  The new supersedes the old  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse, Rat,  (predicted: Human, Dog, Horse, Rabbit, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 25kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human GSTA1/Glutathione S Transferase alpha: 51-150/222 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Members of the glutathione S-transferase (GST) family of proteins function in the detoxification of xenobiotics to protect cells against toxicant-induced damage. GSTs are differentially expressed in lung, liver and kidney tissue. Three isoforms, GSTA1-1, GSTA1-4 and GSTM1, localize to the mitochondria in addition to the cytoplasm. In normal and transformed cells, the oncoprotein Myb transcriptionally upregulates GSTM1. This isoform shows high specific activity for aflatoxin B1 epoxide conjugation, suggesting an important role for this interaction in the defense against both chemical and oxidative stress. The C-terminal domain of GSTA1 may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.

Function:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Subunit:
Homodimer or heterodimer of GSTA1 and GSTA2.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Liver.

Similarity:
Belongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.

SWISS:
P08263

Gene ID:
2938

Database links:

Entrez Gene: 2938 Human

Entrez Gene: 14857 Mouse

Entrez Gene: 24421 Rat

Omim: 138359 Human

SwissProt: P08263 Human

SwissProt: P13745 Mouse

SwissProt: P00502 Rat

Unigene: 446309 Human

Unigene: 467426 Mouse



Product Picture
Sample:
Liver (Rat) Lysate at 40 ug
Primary: Anti- GSTA1 (SL13398R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSTA1) Polyclonal Antibody, Unconjugated (SL13398R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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