Product Name	GAPDHS
Chinese Name	精子3-磷酸甘油醛脱氢酶-2抗体
Alias	GAPDH2; GAPD2; GAPDH 2; GAPDH 2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase, testis specific; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; G3PT_HUMAN; HSD-35.
literatures	Specific References  (4)     |     SL13282R has been referenced in 4 publications. [IF=6.63] Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17  WB ;  Mouse.   PubMed:36513851 [IF=4.068] Ming Li. et al. ADAMTS12, a novel prognostic predictor, promotes cell proliferation, migration and invasion in head and neck squamous cell carcinoma. ORAL DIS. 2022 Oct;:  WB ;  Human.   PubMed:36222542 [IF=2.09] Zhang, Jizheng, Hua Zhang, and Tingting Zi. "Overexpression of microRNA-141 relieves chronic constriction injury-induced neuropathic pain via targeting high-mobility group box 1." International Journal of Molecular Medicine.  WB ;  Rat.   PubMed:26398163 [IF=1.918] Qingxin Fan. et al. Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes. Med Sci Monitor. 2021; 27: e928619-1–e928619-10  WB ;   PubMed:33503016
Research Area	Tumour  Cell biology  Signal transduction  Apoptosis  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GAPDHS: 251-350/408
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein belonging to theglyceraldehyde-3-phosphate dehydrogenase family of enzymes thatplay an important role in carbohydrate metabolism. Like its somaticcell counterpart, this sperm-specific enzyme functions in anicotinamide adenine dinucleotide-dependent manner to removehydrogen and add phosphate to glyceraldehyde 3-phosphate to form1,3-diphosphoglycerate. During spermiogenesis, this enzyme may playan important role in regulating the switch between differentenergy-producing pathways, and it is required for sperm motilityand male fertility. [provided by RefSeq, Jul 2008]. Function: May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility. Subunit: Homotetramer. Interacts with ARRB2; the interaction isdetected in the nucleus upon OR1D2 stimulation. Subcellular Location: Cytoplasm. Tissue Specificity: Testis specific. Similarity: Belongs to the glyceraldehyde-3-phosphatedehydrogenase family. SWISS: O14556 Gene ID: 26330 Database links: Entrez Gene: 26330 Human Entrez Gene: 14447 Mouse Entrez Gene: 66020 Rat Entrez Gene: 532231 Cow Omim: 609169 Human SwissProt: Q2KJE5 Cow SwissProt: O14556 Human SwissProt: Q64467 Mouse SwissProt: Q9ESV6 Rat
Product Picture	Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-GAPDHS (SL13282R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 47 kD Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GAPDHS Polyclonal Antibody, Unconjugated(SL13282R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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